GGEbiplot Pattern Explorer

Complete solution to biplot analysis, video game for scientists


Functionality

GGEbiplot functions

Functions for a 4-Way Dataset

Variable-by-Variable Biplot

Association Biplot

Genotype by env. biplot

Genotype by trait biplot

QTL mapping biplot

QTL by environment biplot

Gene expression biplot

Diallel cross data biplot

Host by pathogen biplot

3D-Biplot

Free User-friendly ANOVA

Breeder's Kit

 

 

Information 

Biplot Analysis Instructions

GGEbiplot Beta download

GGEbiplot License

Biplot Analysis Presentations

User's feedback

GGE biplot publications by users

GGE biplot publications

References on biplot analysis

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  • To see how user-friendly and how powerful GGEbiplot is, try the free test version of GGEbiplot. It is fully functional but is limited to small (7 by 7) datasets.

  • To order GGEbiplot, fill this Order Sheet and send it to us via email. The software will be sent to you, licensed to your name, within ONE day.

Below is a partial description of the GGEbiplot functions. A relatively up-to-date description can be viewed online.


Components

(First year cost)

Functionalities

More advanced descriptions

Basic functions

 

 

Real-time Data Manipulation

  • Allows biplotting transposed data (making it easy for data input and resulting in 3 additional multiplicative models)

  • Allows biplotting of the balanced subset or partially balanced subsets

  • Allows biplotting any subset of the original data by user-specified deleting of any number of the entries and/or testers

  • Go back to a previous subset or the original data

  • Entry and tester stratifications based on vector length, mean value, or biplot position.

  • 4 Missing data filling options.

  • Adding derived variables

3-way data input and visualization

  • Direct input and biplot analyses of genotype by environment by trait three-way data,

  • Generating biplots of all possible two-way tables by selecting any, some, or all of the environments, genotypes, or traits.

  • GGE biplots of any of the traits

  • Genotype by trait biplots in one, some, or all of the environments

  • Trait relationships based on genetic, environmental, and phenotypic data

  • Genotype classification treating each environment-trait as a different variable

  • Other types of biplots

 

4-way data input and visualization

 

  • This adds another dimensional over the three-way data functions. Therefore it makes the 3-way data functions many times more handy

  • Inputs year-location-genotype-trait four-way data from a single file and allows generation and visualization of all possible two-way tables through selecting any, some, or all of the years, locations, genotypes, or traits. In addition to biplot analysis, this module also serves a powerful data manipulation/sub-setting tool.

Multi-way data input and ANOVA

  • Read data from a spreadsheet of any format as soon as the traits are in parallel columns. It can be 3-wy, 4-way, or any way, and the columns of the factors (year, location, site, others) do not have to follow an order.
  • Conduct ANOVA for any number of the factors, across year, across locations, or by year, by location.
  • LSD is calculated and entries ranked on there Mean values divided by LSD, which is a superior way to visually compare multiple entries.

Variable by variable (V-V)  biplot analysis

  • Applicable to studies aiming at understanding the relationships between and within two sets of variables: response variables vs. explanatory variables.

  • QTL identification using multi-environment data; visualizing QTL main effect and specific QTL by environment interactions, which naturally leads to the development of marker based selection strategies specific to different mega-environments.

  • Interpretation of genotype-by-environment interactions using genetic covariables (explanatory traits, genetic markers, QTL, genes, etc.)

  • Conduct congruence analysis, i.e, quantifying the similarity between patterns of two matrices or biplots.

 

Biplot assisted Association Analysis, including correlation and Multiple Regression

  • Identifies associated variables (e.g. markers/traits) for any particular variable (e.g. marker or trait) at user specified significant level with/without removing the effects of one or more covariables (covariate analysis).

  • Any of the variables can be specified as a dependent variable, independent variable, or covariable.

  • This function can be used to identify 1) linked markers, 2) associated traits, 3) QTL identification, 4) pleiotropic effects of a genetic locus, and 5) removing irrelevant variables/markers prior to multiple regression - the last point is essential when there are too many independent variables relative to the number of observations.

  • Results displayed both graphically (in a biplot) and numerically.

  • Conduct multiple regression using any of the variables as the dependent variable and others as independent variables;

  • Delete independent variables from the multiple regression model at the user’s (visual) judgment or based on log ratio (a technique that has been used in QTL mapping), which removes variables that are only indirectly associated with the dependent variable;

  • Generates linear regression model with interaction (digenic epistasis) terms and/or quadratic terms;

  • Results displayed both graphically (in a biplot) and numerically.

3D biplot viewer

  • Visualize a constantly rotating 3D biplot;
  • Adjust rotation speed;
  • Freeze image to output an interesting pattern;
  • Rotate any of the three axes while holding the other two;
  • Visualize 2D biplots of PC1 vs PC2, PC1 vs PC3, and PC2 vs PC3; and
  • Save the current view of the 3D biplot into a graphic file, in color or in black-white
  • Show/hide testers/entries.
  • Show/Hide the vectors.
  • Essential for understanding patterns for large datasets

 


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